|
KMID : 1234420100380010093
|
|
Korean Journal of Microbiololgy and Biotechnology
2010 Volume.38 No. 1 p.93 ~ p.104
|
|
|
The Effect of Nebulized Frankincense Essential Oil in an OVA-Induced Allergic Asthma Mouse Model
|
|
Lee Hye-Youn
Kim Kum-Lan
Kang Sang-Mo
|
|
|
Abstract
|
|
|
|
In this study, we investigated the effects of frankincense essential oil (BSEO) on the immune cell change in the lung, BALF and PBMC using a mouse model of asthma. BALB/c mice after intraperitoneal OVA sensitization (day 1) were challenged intratracheally with OVA on day 14. Then, the asthma was induced by repeated OVA inhalation challenged. The asthma induced mice group inhaled 0.3% BSEO for 30 minutes per trial, three times a week, for 8 weeks using the nebulizer. After 12 weeks from the experiment, the mice was killed and the lung, bronchoalveolar lavage fluid (BALF) and peripheral blood mononuclear cell (PBMC) were obtained. Next, the change of immune cells inside the separated tissues was observed to identify the effects of BSEO on the allergic asthma mice. In conclusion, the hypersensitive reaction of airway to the bronchoconstrictor in the allergic asthma induced mice was effectively suppressed in Frankincense group, in Bermagot, Eucalyptus, Chamomile, Marjoram and Frankincense groups, the natural aromatic essential oil groups. Furthermore, it was also confirmed that the weight of lung, total number of alveolus cells and the number of BALF, MNL and DLN increased after inducing allergic asthma were reduced. BSEO suppressed the percentage of CD3e+/CD19-, B220+/CD23+ and CD11b+/Gr-1+ cells in the lung tissue of allergic asthma mice. Moreover, BSEO also reduced the percentage of CD4+/CD8-, B220+/CD23+ and CD3+/CCR3+ cells in BALF. In addition, the percentage of CD3e+/CD19-, CD3+/CD69+ and B220+/CD23+ cells in PBMC was reduced. The results of this study indicate that BSEO would be effective to treat allergic asthma by the immune control suppressing the activity of immune cells in each tissue.
|
|
|
KEYWORD
|
|
|
Frankincense essential oil, allergic asthma, airway inflammation, eosinophils
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|